CR, June 15, 2001

Combinatorial Chemistry

Outline for today's lecture



Oligonucleotide Libraries, SELEX
 
 

1. Basics of DNA synthesis

2. DNA libraries

3. The SELEX experiment

4. SELEX with modified building blocks
 

key references

1. Ellington, A.D.; Szostak, J.W. Nature 1990, 346, 818-822.

2. Tuerk, C.; Gold, L. Science 1990, 249, 505-510.

3. Gold, L.; Polisky, B.; Uhlenbeck, O.; Yarus, M. Annu. Rev. Biochem. 1995, 64, 763-797.

4. Lee, S.E.; Sidorov, A.; Gourlain, T.; Mignet, N.; Thorpe, S.J.; Bazier, J.A.; Dickman, M.J. Hornby, D.P.; Grasby, J.A.; Williams, D.M. Nucleic Acids Res. 2001, 29, 1565-1573.