CR, May 18, 2001

Combinatorial Chemistry

Outline for today's lecture


Split and pool synthesis

1. Introduction to split-and-pool synthesis of combinatorial libraries

2. Statistics, synthesis, affinity-panning, and sequencing

3. Examples of studies employing split-and-pool libraries for studying peptide-protein interactions
   -  ligands for b-endorphin and streptavidin
   -  ligands for SH3 domains
   -  mapping endoprotease specificity

key references

1. Lam, K.S.; Salmon, S.E.; Hersh, E.M.; Hruby, V.J.; Kazmierski, W.M.; Knapp, R.J. Nature 1991, 354, 82-84.

2. Chen, J.K.; Lane, W.S.; Brauer, A.W.; Tanaka, A.; Schreiber, S.L. J. Am. Chem. Soc. 1993, 115, 12591-12592.

3. Meldal, M.; Svendsen, I.; Breddam, K.; Auzanneau, F.-I. Proc. Natl. Acad. Sci. USA 1994, 91, 3314-3318.