Vertiefungskurs Nucleic Acids Chemistry
Homepage of this course: http://18.104.22.168/NAC_2000/Index.html
Some general guidelines designed to help you prepare for the
- It is not important that you remember the names of investigators, dates or journals, or memorize the exact structures of all intermediates or rare compounds discussed.
- What you should know is principles of nucleic acids chemistry and the syntheses we discussed.
- For example, you should know that purines depurinate under acidic conditions, and that the synthesis of nucleosides from glycosyl donors and a silylated nucleobase is catalyzed by Lewis acids.
- Please go over your notes. The material presented on the board is what is relevant (and chemical and biochemical questions that can be deduced readily from it).
- You need 50% of the points to pass.
The following list highlights portions of the material discussed that are likely to be covered in the final:
The Structure of DNA
Ribose, nucleobase, nucleosides, nucleotides, pKas
DNA and RNA Structure
Watson-Crick base pairing, Hoogsteen base pairing, A- and B-form duplexes, Triple helices,
Chemical Synthesis of Nucleotides
Glycosylation Methods: Vorbrüggen glycosylation; Anchimeric effect and stereoselectivity
Chemical Synthesis of DNA and RNA
Coupling Methods: Phosphotriester method, Phosphoramidite method, Solid-Phase Synthesis of DNA
Biosynthesis of Nucleotides
Biosynthesis of Inosine-5'-phosphate, Conversion of IMP to AMP and GMP, Synthesis of uridine monophosphate
Replication, Transcription and Translation
Chemical Reactions of DNA and RNA
Non-Covalent Binding of Small Molecules to DNA and RNA
Intercalators, Groove binders
Chromatin structure, Zinc fingers
Light-Directed Synthesis of Oligonucleotide Arrays, Synthesis of Oligonucleotide Arrays via Spotting
Phosphoramidates as Phosphodiester-Surrogates, Synthesis of PNAs
Antisense Inhibition of Gene Expression
Gene Expression and the Central Dogma of Molecular Biology, First Generation Antisense Agents, Antigene-Oligonucleotide Inhibitors
Gene Therapy and Chimeraplasty
Categories of gene therapy, Viral and non-viral carriers, Targeted gene correction or chimeraplasty
Fate of dsRNA injected into organisms, need for nuclease(s)