CR, 19. Dezember 2002

Nucleic Acids Chemistry

Outline of Today's Lecture

Microarrays (DNA Chips)

Watching Cellular Gene Activity: Expression Profiling

1.  Principles of DNA Chips

     - spatially addressable arrays of hybridization probes

2.  Light-Directed Synthesis of Oligonucleotide Arrays

     - masks and photolabile protecting groups

3.  Synthesis of Oligonucleotide Arrays via Spotting

     - surface modifications and immobilization methods

4.  Preparation of cDNA Microarrays

     - PCR amplification and spotting