CR, 19. Dezember 2002


Nucleic Acids Chemistry

Outline of Today's Lecture
 
 

Microarrays (DNA Chips)
 

Watching Cellular Gene Activity: Expression Profiling
 

1.  Principles of DNA Chips

     - spatially addressable arrays of hybridization probes
 

2.  Light-Directed Synthesis of Oligonucleotide Arrays

     - masks and photolabile protecting groups
 

3.  Synthesis of Oligonucleotide Arrays via Spotting

     - surface modifications and immobilization methods
 

4.  Preparation of cDNA Microarrays

     - PCR amplification and spotting