CR, 4. Mai 2005

Nucleic Acids Chemistry

Outline of Today's Lecture
 

1.  Structure of Triple Helices

     -  Base pairing and strand orientations

     -  H-DNA
 
 

2. Chemical Synthesis of DNA and RNA Oligomers
 

2.1  Retrosynthetic Considerations

2.2  Chain Extension Methods

     -  Phosphotriester method

     -  H-Phosphonate method

     -  Phosphoramidite method
 

3.  Solid-Phase Synthesis of DNA
 

4.  Basics of the chemical synthesis of RNA
 

Reference: Chapter 3 in Blackburn & Gait