CR, 11. Mai 2005

Nucleic Acids Chemistry

Outline of Today's Lecture
 

Biosynthesis of DNA and RNA - Replication & Transcription
 

1. The Central Dogma Of Molecular Biology
 

2. Replication, catalyzed by a DNA-polymerase

     -  Extension reaction

     -  Polymerases

     -  Prokaryotic replisome

     -  Ligase reaction
 

3. Transcription, the synthesis of RNA

     -  Chemical aspects

     -  Preinitiation complex and regulation of gene expression
 

4. Synthesis of Triphosphates

     -  Ötvös method
 

References: chapters 30, 31 in Voet and Voet, chapter 8 in Watson