CR, June 22, 2005

Nucleic Acids Chemistry

Outline of Today's Lecture



Microarrays (DNA Chips)
 

1.  Principles of DNA chips

     -  Microarrays of hybridization probes

     -  Massively parallel gene expression analysis
 

2.  Microarrays via photolabile protecting groups

     -  Principles of mask-based synthesis

     -  Mechanism of removal of UV-labile PGs
 

3.  Synthesis of oligonucleotide arrays with the aid of UV light and photogenerated acids

     -  Synthetic approach

     -  Photogenerated acids
 

4.  Preparing microarrays via spotting

     -  Synthesis of probes or cDNAs

     -  Immobilization strategies
 

Selected references: 1) Fodor et al. Science 1991, 251, 767.  2) Brown et al. Science 1995, 270, 467.
3) Gao et al. Nucleic Acids Res. 2001, 29, 4744.  4) Pirrung, Angew. Chem. 2002, 114, 1326 (review).