CR, February 1, 2007

Nucleic Acids Chemistry

Outline of Today's Lecture

12. Microarrays (DNA Chips)

12.1  Principles of DNA chips

     -  Microarrays of hybridization probes

     -  Massively parallel gene expression analysis

12.2   Microarrays via photolabile protecting groups

     -  Principles of mask-based synthesis

     -  Mechanism of removal of UV-labile PGs

12.3   Preparing microarrays via spotting

     -  Synthesis of probes or cDNAs

     -  Immobilization strategies

12.4  Synthesis of oligonucleotide arrays with the aid of UV light and photogenerated acids

     -  Synthetic approach

     -  Photogenerated acids

                 Pirrung, Angew. Chem. 2002, 114, 1326.