CR, 14.12.2006

Nucleic Acids Chemistry

Outline of Today's Lecture
 
 

6. Non-covalent DNA- and RNA-Binding

6.1  Small Molecule Ligands

    - Overview of binding modes to duplexes

    - Electrostatic binding to the backbone
 
        Polyamines, effect of salt concentration on UV melting points

    - Intercalators
 
        Structures of stains and drugs that intercalate

    - Groove binders

        Natural products that are minor groove binders

        Pairing rules for sequence-specific polyamides
 

6.2  Protein Ligands

    - Transcriptional control: Zinc fingers & leucine zippers

    - alpha-helices binding in the major groove
 
 

References:

    - Chapter 8 &9 in Blackburn & Gait (chapter 9 & 10 in 3rd edition)
    - Dervan & Edelson, Curr. Opin. Struc. Biol. 2003, 13, 284-299.