CR, 16. November 2006

Nucleic Acids Chemistry


Outline of Today's Lecture
 

4. Chemical Synthesis of Nucleoside Triphosphates, Replication, Transcription
 

4.1 Synthesis of Triphosphates from Nucleosides

     -  Phosphorylation of nucleosides

     -  Ötvös method for preparation of triphosphates
 

4.2 Replication & Transcription

     -  The central dogma of molecular biology

     -  Replication, catalyzed by a DNA-polymerase

     -  Extension reaction
 

3. Transcription, the synthesis of RNA

     -  Chemical aspects

     -  Preinitiation complex and regulation of gene expression
 

References:
  - Chapters 30, 31 in Voet & Voet
  - Chapter 3 of Blackburn & Gait