Nucleic Acids Chemistry
Outline of Today's Lecture
9.1 Comparison with Peptide Sequencing
9.2 Maxam-Gilbert Method
- Base-specific fragmentation
- Separation of strands by PAGE
9.3 Dideoxy- or Chain Terminator Method
- 2',3'-Dideoxynucleotide triphosphates
- Synthesis of truncated products
9.4 Automated 1 Reaction/4 Fluorophore Method
- Big Dye-Labeled ddNTPs
- Read-out by capillary electrophoresis
- Section 28-6, Voet & Voet, Biochemistry 2nd ed.
- Section 7-2, Voet & Voet, Biochemistry 3rd ed.