CR, Januar 18, 2007

Nucleic Acids Chemistry


Outline of Today's Lecture
 

9. DNA-Sequencing

9.1  Comparison with Peptide Sequencing

9.2  Maxam-Gilbert Method

     -  Base-specific fragmentation

     -  Separation of strands by PAGE
 

9.3  Dideoxy- or Chain Terminator Method

     -  2',3'-Dideoxynucleotide triphosphates

     -  Synthesis of truncated products
 

9.4  Automated 1 Reaction/4 Fluorophore Method

     -  Big Dye-Labeled ddNTPs

     -  Read-out by capillary electrophoresis
 

References
- Section 28-6, Voet & Voet, Biochemistry 2nd ed.
- Section 7-2, Voet & Voet, Biochemistry 3rd ed.