CR, 23. November 2006

Nucleic Acids Chemistry

Outline of Today's Lecture
 

5. Chemical Synthesis of DNA 
 

5.1  Retrosynthetic Considerations
 

5.2  Coupling methods

     -  Phosphotriester method

     -  H-Phosphonate method

     -  Phosphoramidite method
 

5.3  Protecting groups and solid support

     -  Phosphate protecting groups

     -  DMT as 5'-protecting group

     -  Acyl protecting groups for nucleobases

     -  Long chain alkylamine controlled pore glass
 

5.4  Solid-Phase Synthesis of DNA

     -  Chain extension cycle

     -  Deprotection and liberation from support
 

Reference: Chapter 3 in Blackburn & Gait