CR, Janauary 25, 2007

Nucleic Acids Chemistry


Outline of Today's Lecture
 

10. Genotyping, Epigenomic Analysis and Ligation Assays
 

1.  Variances in the Genome

     -  Single Nucleotide Polymorphisms

     -  Mutations

     -  Haplotypes and the HapMap Project
 

2.  Determining SNP Alleles (Genotyping)

     -  Sequence-specific hybridization

     -  Primer extension assay

     -  Taqman Assay
 

4.  The Epigenome

     -  Methylation of dC affects gene expression

     -  Bisulfite-based methylation assay